High affinity sugar ligands of C-type lectin receptor langerin

Fumi Ota, Tetsuya Hirayama, Yasuhiko Kizuka, Yoshiki Yamaguchi, Reiko Fujinawa, Masahiro Nagata, Hendra S. Ismanto, Bernd Lepenies, Jonas Aretz, Christoph Rademacher, Peter H. Seeberger, Takashi Angata, Shinobu Kitazume, Keiichi Yoshida, Tomoko Betsuyaku, Kozui Kida, Sho Yamasaki, Naoyuki Taniguchi

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Background: Langerin, a C-type lectin receptor (CLR) expressed in a subset of dendritic cells (DCs), binds to glycan ligands for pathogen capture and clearance. Previous studies revealed that langerin has an unusual binding affinity toward 6-sulfated galactose (Gal), a structure primarily found in keratan sulfate (KS). However, details and biological outcomes of this interaction have not been characterized. Based on a recent discovery that the disaccharide L4, a KS component that contains 6-sulfo-Gal, exhibits anti-inflammatory activity in mouse lung, we hypothesized that L4-related compounds are useful tools for characterizing the langerin-ligand interactions and their therapeutic application. Methods: We performed binding analysis between purified long and short forms of langerin and a series of KS disaccharide components. We also chemically synthesized oligomeric derivatives of L4 to develop a new high-affinity ligand of langerin. Results: We show that the binding critically requires the 6-sulfation of Gal and that the long form of langerin displays higher affinity than the short form. The synthesized trimeric (also designated as triangle or Tri) and polymeric (pendant) L4 derivatives displayed over 1000-fold higher affinity toward langerin than monomeric L4. The pendant L4, but not the L4 monomer, was found to effectively transduce langerin signaling in a model cell system. Conclusions: L4 is a specific ligand for langerin. Oligomerization of L4 unit increased the affinity toward langerin. General significance: These results suggest that oligomeric L4 derivatives will be useful for clarifying the langerin functions and for the development of new glycan-based anti-inflammatory drugs.

本文言語English
ページ(範囲)1592-1601
ページ数10
ジャーナルBiochimica et Biophysica Acta - General Subjects
1862
7
DOI
出版ステータスPublished - 2018 7

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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