High-contrast fluorescence imaging based on the polarization dependence of the fluorescence enhancement using an optical interference mirror slide

Mitsuru Yasuda, Takuo Akimoto

研究成果: Article査読

3 被引用数 (Scopus)

抄録

High-contrast fluorescence imaging using an optical interference mirror (OIM) slide that enhances the fluorescence from a fluorophore located on top of the OIM surface is reported. To enhance the fluorescence and reduce the background light of the OIM, transverse-electric-polarized excitation light was used as incident light, and the transverse-magnetic-polarized fluorescence signal was detected. As a result, an approximate 100-fold improvement in the signal-to-noise ratio was achieved through a 13-fold enhancement of the fluorescence signal and an 8-fold reduction of the background light.

本文言語English
ページ(範囲)139-143
ページ数5
ジャーナルanalytical sciences
31
3
DOI
出版ステータスPublished - 2015
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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