High efficiency gene delivery into laryngeal muscle with bidirectional electroporation

Koichiro Saito, Mohamed Lehar, Zhao Bo Li, Natasha Braga, Alan D. King, Robin A. Samlan, Paul W. Flint

研究成果: Article

5 引用 (Scopus)

抜粋

Objective: The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle. Study design and setting: High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional). Results: HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation. Conclusion: HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma. Significance: Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.

元の言語English
ページ(範囲)209-214
ページ数6
ジャーナルOtolaryngology - Head and Neck Surgery
135
発行部数2
DOI
出版物ステータスPublished - 2006 8 6

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

フィンガープリント High efficiency gene delivery into laryngeal muscle with bidirectional electroporation' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Saito, K., Lehar, M., Li, Z. B., Braga, N., King, A. D., Samlan, R. A., & Flint, P. W. (2006). High efficiency gene delivery into laryngeal muscle with bidirectional electroporation. Otolaryngology - Head and Neck Surgery, 135(2), 209-214. https://doi.org/10.1016/j.otohns.2006.04.003