High-resolution fluorescence microscopy based on a cyclic sequential multiphoton process

Keisuke Isobe, Akira Suda, Hiroshi Hashimoto, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa

研究成果: Article査読

11 被引用数 (Scopus)

抄録

We demonstrate high-resolution fluorescence microscopy based on a cyclic sequential multiphoton (CSM) process, which gives rise to fluorescence emission following a sequence of cyclic transitions between the bright and dark states of a fluorophore induced by pump and reverse light. By temporally modulating the reverse intensity, we can extract the fluorescence signal generated through the CSM process. We show that the demodulated fluorescence signal is nonlinearly proportional to the excitation intensities and it gives a higher spatial resolution than that of a confocal microscope.

本文言語English
ページ(範囲)791-797
ページ数7
ジャーナルBiomedical Optics Express
1
3
DOI
出版ステータスPublished - 2010 10月 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • 原子分子物理学および光学

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