High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes

Naoko Goto-Inoue, Takahiro Hayasaka, Yuki Sugiura, Takao Taki, Yu Teh Li, Mineo Matsumoto, Mitsutoshi Setou

研究成果: Article査読

51 被引用数 (Scopus)

抄録

Glycosphingolipids are ubiquitous constituents of cells. Yet there is still room for improvement in the techniques for analyzing glycosphingolipids. Here we report our highly sensitive and convenient analytical technology with imaging mass spectrometry for detailed structural analysis of glycosphingolipids. We were able to determine detailed ceramide structures; i.e., both the sphingosine base and fatty acid, by MS/MS/MS analysis on a PVDF membrane with 10 pmol of GM1, with which only faint bands were visible by primuline staining. The limit of detection was approximately 1 pmol of GM1, which is lower than the value in the conventional reports (10 pmol).

本文言語English
ページ(範囲)74-83
ページ数10
ジャーナルJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
870
1
DOI
出版ステータスPublished - 2008 7月 1
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 臨床生化学
  • 細胞生物学

フィンガープリント

「High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル