High-speed super-multiplex imaging of brain tissue

Robert Oda, Jingwen Shou, Keiko Karasawa, Mutsuo Nuriya, Masato Yasui, Yasuyuki Ozeki

研究成果: Conference contribution

抄録

Cells within the brain are highly organized and coordinate complex processes with each other. The ability to simultaneously visualize the organization and interactions of cells and molecules within brain tissue remains an important issue to understand the brain comprehensively. Stimulated Raman scattering (SRS) and fluorescence, two powerful imaging modalities, can provide complementary molecular contrasts within cells and tissue samples. Here, we present a high-speed super-multiplex imaging platform that combines SRS microscopy with confocal fluorescence microscopy to perform rapid 7-color brain imaging. We show simultaneous imaging of cellular components within the brain such as astrocytes, axons, and blood vessels while also showing organelles such as the nucleus and actin cytoskeleton. Also, we demonstrate the ability to take depth-resolved images that elucidate the three-dimensional organization of diverse components within brain tissue. This platform can be adapted to explore various processes within brain tissue that can reveal critical information about the brain and how it is affected by diseases, which leads toward a deeper understanding of disease progression and potentially the development of therapeutic options for brain diseases.

本文言語English
ホスト出版物のタイトルAdvanced Chemical Microscopy for Life Science and Translational Medicine 2021
編集者Ji-Xin Cheng, Wei Min, Garth J. Simpson
出版社SPIE
ISBN(電子版)9781510641471
DOI
出版ステータスPublished - 2021
イベントAdvanced Chemical Microscopy for Life Science and Translational Medicine 2021 - Virtual, Online, United States
継続期間: 2021 3 62021 3 11

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
11656
ISSN(印刷版)1605-7422

Conference

ConferenceAdvanced Chemical Microscopy for Life Science and Translational Medicine 2021
国/地域United States
CityVirtual, Online
Period21/3/621/3/11

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 原子分子物理学および光学
  • 生体材料
  • 放射線学、核医学およびイメージング

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