High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization

Toshiyuki Yoshikawa, Yulan Piao, Jinhui Zhong, Ryo Matoba, Mark G. Carter, Yuxia Wang, Ilya Goldberg, Minoru S.H. Ko

研究成果: Article査読

68 被引用数 (Scopus)

抄録

Mammalian preimplantation embryos provide an excellent opportunity to study temporal and spatial gene expression in whole mount in situ hybridization (WISH). However, large-scale studies are made difficult by the size of the embryos (∼60 μm diameter) and their fragility. We have developed a chamber system that allows parallel processing of embryos without the aid of a microscope. We first selected 91 candidate genes that were transcription factors highly expressed in blastocysts, and more highly expressed in embryonic (ES) than in trophoblast (TS) stem cells. We then used the WISH to identify 48 genes expressed predominantly in the inner cell mass (ICM) and to follow several of these genes in all seven preimplantation stages. The ICM-predominant expressions of these genes suggest their involvement in the pluripotency of embryonic cells. This system provides a useful tool to a systematic genome-scale analysis of preimplantation embryos.

本文言語English
ページ(範囲)213-224
ページ数12
ジャーナルGene Expression Patterns
6
2
DOI
出版ステータスPublished - 2006 1月
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学
  • 発生生物学

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