High-yield selection and extraction of two promoter-defined phenotypes of neural stem cells from the fetal human brain

H. Michael Keyoung, Neeta S. Roy, Abdellatif Benraiss, Abner Louissaint, Akira Suzuki, Mitsuhiro Hashimoto, William K. Rashbaum, Hideyuki Okano, Steven A. Goldman

研究成果: Article査読

145 被引用数 (Scopus)

抄録

Neural stem and precursor cells reside in the ventricular lining of the fetal forebrain, and may provide a cellular substrate for brain repair. To selectively identify and extract these cells, we infected dissociated fetal human brain cells with adenoviruses bearing the gene for green fluorescence protein (GFP), placed under the control of enhancer/promoters for two genes (nestin and musashil) that are expressed in uncommitted neuroepithelial cells. The cells were then sorted by fluorescence-activated cell sorting (FACS) on the basis of E/nestin- or P/musashi1-driven GFP expression. Both P/musashi1:hGFP- and E/nestin:EGFP-sorted cells were multipotent: limiting dilution with clonal expansion as neurospheres, in tandem with retroviral lineage analysis and xenograft to E17 and P0-2 rat forebrain, revealed that each phenotype was able to both self-renew and co-generate neurons and glia. Thus, fluorescent genes placed under the control of early neural promoters allow neural stem cells to be specifically targeted, isolated, and substantially enriched from the fetal human brain.

本文言語English
ページ(範囲)843-850
ページ数8
ジャーナルNature Biotechnology
19
9
DOI
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー
  • 分子医療
  • 生体医工学

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