Highly sensitive detection of influenza virus by boron-doped diamond electrode terminated with sialic acid-mimic peptide

研究成果: Article査読

33 被引用数 (Scopus)

抄録

The progression of influenza varies according to age and the presence of an underlying disease; appropriate treatment is therefore required to prevent severe disease. Anti-influenza therapy, such as with neuraminidase inhibitors, is effective, but diagnosis at an early phase of infection before viral propagation is critical. Here, we show that several dozen plaque-forming units (pfu) of influenza virus (IFV) can be detected using a boron-doped diamond (BDD) electrode terminated with a sialic acid-mimic peptide. The peptide was used instead of the sialyloligosaccharide receptor, which is the common receptor of influenza A and B viruses required during the early phase of infection, to capture IFV particles. The peptide, which was previously identified by phage-display technology, was immobilized by click chemistry on the BDD electrode, which has excellent electrochemical characteristics such as low background current and weak adsorption of biomolecules. Electrochemical impedance spectroscopy revealed that H1N1 and H3N2 IFVs were detectable in the range of 20-500 pfu by using the peptide-terminated BDD electrode. Our results demonstrate that the BDD device integrated with the receptor-mimic peptide has high sensitivity for detection of a low number of virus particles in the early phase of infection.

本文言語English
ページ(範囲)8981-8984
ページ数4
ジャーナルProceedings of the National Academy of Sciences of the United States of America
113
32
DOI
出版ステータスPublished - 2016 8 9

ASJC Scopus subject areas

  • 一般

フィンガープリント

「Highly sensitive detection of influenza virus by boron-doped diamond electrode terminated with sialic acid-mimic peptide」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル