TY - JOUR
T1 - hnRNP-U enhances the expression of specific genes by stabilizing mRNA
AU - Yugami, Masato
AU - Kabe, Yasuaki
AU - Yamaguchi, Yuki
AU - Wada, Tadashi
AU - Handa, Hiroshi
N1 - Funding Information:
We thank Yohei Amano and Masafumi Suzuki for technical assistance. This work was supported in part by Grant-in-Aid for Scientific Research on Priority Areas, the Special Coordination Funds for Promoting Science and Technology of the Ministry of Education and the Grant of the 21st Century COE Program from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. This work was also supported by a grant from the New Energy and Industrial Technology Development Organization, Japan to H.H. M.Y. is a JSPS research fellow.
PY - 2007/1/9
Y1 - 2007/1/9
N2 - Heterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre-mRNA processing. hnRNP-U, also termed scaffold attachment factor A (SAF-A), binds to pre-mRNA and nuclear matrix/scaffold attachment region DNA elements. However, its role in the regulation of gene expression is as yet poorly understood. In the present study, we show that hnRNP-U specifically enhances the expression of tumor necrosis factor α mRNA by increasing its stability, possibly through binding to the 3′ untranslated region. We also show that hnRNP-U enhances the expression of several other genes as well, including GADD45A, HEXIM1, HOXA2, IER3, NHLH2, and ZFY, by binding to and stabilizing these mRNAs. These results suggest that hnRNP-U enhances the expression of specific genes by regulating mRNA stability.
AB - Heterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre-mRNA processing. hnRNP-U, also termed scaffold attachment factor A (SAF-A), binds to pre-mRNA and nuclear matrix/scaffold attachment region DNA elements. However, its role in the regulation of gene expression is as yet poorly understood. In the present study, we show that hnRNP-U specifically enhances the expression of tumor necrosis factor α mRNA by increasing its stability, possibly through binding to the 3′ untranslated region. We also show that hnRNP-U enhances the expression of several other genes as well, including GADD45A, HEXIM1, HOXA2, IER3, NHLH2, and ZFY, by binding to and stabilizing these mRNAs. These results suggest that hnRNP-U enhances the expression of specific genes by regulating mRNA stability.
KW - 3′ Untranslated region
KW - Gene expression
KW - Heterogeneous ribonucleoprotein
KW - Tumor necrosis factor
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U2 - 10.1016/j.febslet.2006.11.062
DO - 10.1016/j.febslet.2006.11.062
M3 - Article
C2 - 17174306
AN - SCOPUS:33845642707
SN - 0014-5793
VL - 581
SP - 1
EP - 7
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -