HTLV-1 p27(rex) stabilizes human interleukin-2 receptor α chain mRNA

H. Kanamori, N. Suzuki, H. Siomi, T. Nosaka, A. Sato, H. Sabe, M. Hatanaka, T. Honjo

研究成果: Article査読

37 被引用数 (Scopus)

抄録

Expression of the pX gene products (p40(tax), p27(rex) and p21(X-III) of human T cell leukemia virus type 1 (HTLV-1), which is known to be a causative agent of adult T cell lymphoma/leukemia, induces expression of the interleukin-2 receptor α chain (IL-2Rα) on infected T cells. Comparison of IL-2Rα promoter activities has revealed that the transcriptional activation of the promoter alone cannot explain the large numbers of IL-2Rα expressed on HTLV-1 infected cells. We found that the rates of the IL-2Rα mRNA degradation were greatly reduced in pX-positive cells as compared with pX-negative cells. Simultaneous transfection of the expression vector plasmid containing IL-2Rα cDNA and similar plasmids containing various pX sequences showed that p27(rex) elongated the half life of IL-2Rα mRNA. As p27(rex) did not affect the transport of the IL-2Rα mRNA from nucleus to cytoplasm, prolongation of the IL-2Rα mRNA half life by p27(rex) is ascribed to stabilization of the mRNA. Experiments using deletion mutants and chimeric constructs of the IL-2Rα cDNA demonstrated that the coding sequence but not the 5' or 3' untranslated region of the IL-2Rα mRNA sequence is responsible for its protection by p27(rex).

本文言語English
ページ(範囲)4161-4166
ページ数6
ジャーナルEMBO Journal
9
12
DOI
出版ステータスPublished - 1990
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)
  • 分子生物学
  • 生化学、遺伝学、分子生物学(全般)
  • 免疫学および微生物学(全般)

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