Human β nerve growth factor obtained from a baculovirus expression system has potent in vitro and in vivo neurotrophic activity

Jim Barnett, Preston Baecker, Carol Routledge-Ward, Hela Bursztyn-Pettegrew, Joan Chow, Binh Nguyen, Chinh Bach, Hardy Chan, Mark H. Tuszynski, Kazunari Yoshida, Rafael Rubalcava, Fred H. Gage

研究成果: Article査読

40 被引用数 (Scopus)

抄録

A baculovirus expression vector, which contains the coding sequences for human prepro (β) nerve growth factor under control of the viral polyhedrin promoter, was constructed. Upon infection of insect cells with the recombinant virus, mature human β nerve growth factor (rhNGF) was released into the culture fluid. The mature rhNGF was biologically active since rat pheochromocytoma (PC12) and human neuroblastoma (SH-SY5Y) cells were induced to extend neurites upon treatment with this material. This activity was abolished by treating with antiserum prepared against mature mouse β NGF (mNGF). When compared with mNGF, rhNGF more rapidly elicited the differentiation response in both PC12 and SH-SY5Y cells. In an in vivo assay of cholinergic cell survival, rhNGF was nearly as potent as mNGF in protecting cholinergic neurons from degeneration following a fimbria-fornix lesion. These results show that the baculovirus expression system provides quantities of biologically potent human β NGF suitable for a comprehensive program of research to ascertain β NGF's potential as a therapeutic agent for the treatment of Alzheimer's disease.

本文言語English
ページ(範囲)11-24
ページ数14
ジャーナルExperimental Neurology
110
1
DOI
出版ステータスPublished - 1990 10
外部発表はい

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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