Human CD14+ macrophages in intestinal lamina propria exhibit potent antigen-presenting ability

Nobuhiko Kamada, Tadakazu Hisamatsu, Haruki Honda, Taku Kobayashi, Hiroshi Chinen, Mina Tokutake Kitazume, Tetsuro Takayama, Susumu Okamoto, Kazutaka Koganei, Akira Sugita, Takanori Kanai, Toshifumi Hibi

研究成果: Article査読

95 被引用数 (Scopus)

抄録

Intestinal APCs are considered critical in maintaining the balance between the response against harmful pathogens and the induction of tolerance to commensal bacteria and food Ags. Recently, several studies indicated the presence of gut-specific APC subsets, which possess both macrophage and dendritic cell (DC) markers. These unique APC subsets play important roles in gut immunity, especially for immune regulation against commensal bacteria. Herein, we examined a unique macrophage subset, which coexpressed the macrophage (Mφ) marker CD14 and the DC marker CD209 in human intestinal lamina propria (LP). The LP Mφ subset in both normal control subjects or Crohn's disease (CD) patients induced proliferation of naive CD4+ T cells as well as monocyte-derived DCs, and it expressed retinoic acid synthetic enzyme retinaldehyde dehydrogenase 2 and retinol dehydrogenase 10, which induced expression of gut homing receptors on T cells in a retinoic acid-dependent manner. Moreover, the LP Mφ subset strongly evoked differentiation of Th1 cells and slightly induced Th17 cells in both normal control subjects and CD patients; the inducing potential was highest in CD patients. In CD patients, Th17, but not Th1, induction by the LP Mφ subset was enhanced in the presence of commensal bacteria Ags. This enhancement was not observed in normal control subjects. The Th17 induction by the LP Mφ subset was inhibited by neutralization of IL-6 and IL-1β, but it was enhanced by blockade of retinoic acid signaling. These observations highlight a role for LPMφ in the enhanced Th1, and potentially in Th17 differentiation, at the inflammatory site of inflammatory bowel diseases.

本文言語English
ページ(範囲)1724-1731
ページ数8
ジャーナルJournal of Immunology
183
3
DOI
出版ステータスPublished - 2009 8月 1

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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