Human Herpesvirus 6 Reactivation Evaluated by Digital Polymerase Chain Reaction and Its Association with Dynamics of CD134-Positive T Cells after Allogeneic Hematopoietic Stem Cell Transplantation

Hitomi Nakayama, Rie Yamazaki, Jun Kato, Yuya Koda, Masatoshi Sakurai, Ryohei Abe, Shintaro Watanuki, Chieko Sumiya, Kohei Shiroshita, Shinya Fujita, Kentaro Yamaguchi, Shinichiro Okamoto, Takehiko Mori

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Background: Human herpesvirus 6 (HHV-6) causes life-threatening central nervous system disorders after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Recent studies implicated CD134 as a specific receptor of HHV-6B and demonstrated that its expression levels in CD4-positive T cells after allo-HSCT could be related to the reactivation of HHV-6. We prospectively evaluated the relationship between HHV-6 reactivation and CD134+ T cells in the recipients of allo-HSCT. Methods: HHV-6 viral load in plasma was quantitatively measured weekly after allo-HSCT by digital polymerase chain reaction in 34 patients. The ratio of CD134 in CD4+ T cells (CD134/CD4 ratio) was serially measured by flow cytometry before and after transplantation. Results: HHV-6 reactivation was detected in 23 patients (68%). The CD134/CD4 ratio before conditioning was significantly higher in patients with HHV-6 reactivation than in those without (median, 3.8% vs 1.5%, P <. 01). In multivariate analysis, a higher CD134/CD4 ratio before conditioning was significantly associated with the incidence of HHV-6 reactivation (odds ratio, 10.5 [95% confidence interval, 1.3-85.1], P =. 03). Conclusions: A higher CD134/CD4 ratio before conditioning was associated with a higher risk of HHV-6 reactivation, suggesting that the rate may be a promising marker for predicting HHV-6 reactivation after allo-HSCT.

本文言語English
ページ(範囲)1001-1007
ページ数7
ジャーナルJournal of Infectious Diseases
220
6
DOI
出版ステータスPublished - 2019 8 9

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 感染症

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