Human intestinal epithelial cell-derived interleukin (IL)-18, along with IL2, IL-7 and IL-15, is a potent synergistic factor for the proliferation of intraepithelial lymphocytes

A. Okazawa, T. Kanai, K. Nakamaru, T. Sato, N. Inoue, H. Ogata, Y. Iwao, M. Ikeda, T. Kawamura, S. Makita, K. Uraushihara, R. Okamoto, M. Yamazaki, M. Kurimoto, H. Ishii, M. Watanabe, T. Hibi

研究成果: Article査読

47 被引用数 (Scopus)

抄録

Intestinal epithelial cell (IEC)-derived cytokines, such as stem cell factor (SCF), interleukin (IL)-7 and IL-15 are known to be required for the development of intestinal intraepithelial lymphocytes (IELs). A newly described cytokine, IL-18, has also been shown to be produced by intestinal epithelial cells. To demonstrate the functional effects of IL-18 on human IELs, we assessed IL-18/IL-18 receptor expression in IEC/IEL and proliferation following stimulation of intestinal IELs by IL-18. IL-18 transcripts were detected both in freshly isolated human colonie epithelial cells and in various colonie epithelial cell lines. IL-18 protein was also detected by ELISA and flow cytometric analysis using antihuman IL-18-specific monoclonal antibody (MoAb). Furthermore, IELs constitutively expressed the IL-18 receptor in addition to the IL-2 and IL-7 receptors. More importantly, IL-18 augmented significant proliferative responses of IEL in combination with IL-2, IL-7 and IL-15 both in the presence and in absence of anti-CD3 MoAb. These results suggest that IL-18 might play a crucial role in the proliferation and maintenance of intestinal IELs.

本文言語English
ページ(範囲)269-276
ページ数8
ジャーナルClinical and Experimental Immunology
136
2
DOI
出版ステータスPublished - 2004 5
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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