Human rasGTPase-activating protein (human counterpart of GAP1m): Sequence of the cDNA, primary structure of the protein, production and chromosomal localization

Mariko Kobayashi, Tohru Masui, Jun Kusuda, Yosuke Kameoka, Katsuyuki Hashimoto, Shintaro Iwashita

研究成果: Article査読

6 被引用数 (Scopus)

抄録

When cDNA encoding rat rasGTPase-activating protein (rat GAP1m) was used as a probe, two partial cDNA clones of a human counterpart of rat GAP1m were isolated from a cDNA library derived from growth-arrested normal human ectocervical epithelial cells. One clone was found to be a cDNA of premature mRNA with two introns. A complete cDNA of human GAP1m was constructed by a series of reverse transcription-polymerase chain reaction (RT-PCR) using total RNA from human epidermoid carcinoma A431 cells. Human GAP1m shows 87.7% nucleotide identity to rat GAP1m in open reading frame and encodes an 850-amino acid protein that shows 89.2% identity to rat GAP1m. A 100-kDa protein was detected in A431 cells by Western blotting with anti-rat GAP1m antibody. The human GAP1m gene was mapped to chromosome 3q24-q26.

本文言語English
ページ(範囲)173-177
ページ数5
ジャーナルGene
175
1-2
DOI
出版ステータスPublished - 1996 10月 10

ASJC Scopus subject areas

  • 遺伝学

フィンガープリント

「Human rasGTPase-activating protein (human counterpart of GAP1m): Sequence of the cDNA, primary structure of the protein, production and chromosomal localization」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル