Identification of a pathogenic isolate-specific 30,000-M(r) antigen of Entamoeba histolytica by using a monoclonal antibody

H. Tachibana, S. Kobayashi, Y. Kato, K. Nagakura, Y. Kaneda, T. Takeuchi

研究成果: Article査読

66 被引用数 (Scopus)

抄録

A monoclonal antibody (MAb) produced against trophozoites of Entamoeba histolytica strain HM-1:IMSS, reacted with all of 42 isolates and 4 clones showing pathogenic zymodeme (Z) patterns, i.e., Z-II, Z-IIα-, Z-II (glucose phosphate isomerase:γ+), Z-VII, Z-VII (glucose phosphate isomerase:α lack, γ+), Z-XI, Z-XIV, and Z-XIX, regardless of culture conditions, geographical origins, or host symptoms in an indirect fluorescence antibody test. In contrast, the MAb failed to react with 14 isolates possessing nonpathogenic zymodemes Z-I and Z-VIII and did not react with other enteric protozoan parasites, such as E. histolytica-like Laredo, Entamoeba hartmanni, Entamoeba coli, Endolimax nana, Dientamoeba fragilis, Trichomonas hominis, and Giardia lamblia. Western immunoblotting analysis showed that the molecular weight of the antigenic component recognized by the MAb was exclusively 30,000 in pathogenic isolates of different zymodemes. These results suggest that the 30,000-molecular-weight antigen is a marker of pathogenic isolates and that the indirect fluorescent-antibody test with the MAb is useful for the accurate discrimination of pathogenic amebae.

本文言語English
ページ(範囲)955-960
ページ数6
ジャーナルInfection and Immunity
58
4
出版ステータスPublished - 1990
外部発表はい

ASJC Scopus subject areas

  • 寄生虫科
  • 微生物学
  • 免疫学
  • 感染症

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