Identification of mecA gene in MRSA by multiplex PCR

S. Hara, E. Hayashi, H. Imazeki, H. Ohtani, A. Nakata, T. Muramatsu, S. Higuchi

研究成果: Article査読

抄録

Methicillin-Resistant Strains of Staphylococci (MRSA) remains an important pathogen of hospital infection and the number of affected patients is rising. To limit the transmission of these organisms, early detection is crucial and Polymerase Chain Reaction (PCR) is considered to be a powerful tool for this purpose. A primary concern with PCR-based studies is whether amplified DNA may be derived from laboratory contaminants. On the contrary, negative results may be attributed to amplification errors. To overcome these problems, we developed a multiplex PCR assay using oligonucleotide primers to detect mecA and 16S ribosomal RNA gene. The results are accordant with conventional disk method or MIC method, suggesting multiplex PCR to be a useful measure for identification of MRSA.

本文言語English
ページ(範囲)470-474
ページ数5
ジャーナルIRYO - Japanese Journal of National Medical Services
52
8
出版ステータスPublished - 1998
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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