Identification of mouse piRNA pathway components using anti-MIWI2 antibodies

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The mouse testis has served as a popular model system to study a wide range of biological processes, including germcell development, meiosis, epigenetic changes of chromatin, transposon silencing, and small RNA-mediated epigenetic modifications. PIWI-interacting RNAs (piRNAs) are a class of small RNAs that are almost exclusively expressed in animal gonads. They repress transposons by forming effector complexes with PIWI proteins to maintain genome integrity of the germline. Here we describe detailed procedures of how to produce monoclonal antibodies against a mouse nuclear PIWI protein, MIWI2, which functions in de novo DNA methylation of target transposon loci. We then describe how to use the antibodies to isolate associated complexes and to detect MIWI2 immunohistochemically.

元の言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版者Humana Press Inc.
ページ205-216
ページ数12
DOI
出版物ステータスPublished - 2017

出版物シリーズ

名前Methods in Molecular Biology
1463
ISSN(印刷物)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • これを引用

    Hirano, T., Hasuwa, H., & Siomi, H. (2017). Identification of mouse piRNA pathway components using anti-MIWI2 antibodies. : Methods in Molecular Biology (pp. 205-216). (Methods in Molecular Biology; 巻数 1463). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-4017-2_15