Identification of novel in vivo obligate GroEL/ES substrates based on data from a cell-free proteomics approach

Tatsuya Niwa, Kei Fujiwara, Hideki Taguchi

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Chaperones are essential to maintain the proper folding of various proteins in vivo. The Escherichia coli chaperonin GroEL/GroES (GroE) is one of the best-studied chaperones, and its in vivo substrates have been identified, mainly by mass spectrometry-based proteomic studies. Here, we newly identified 20 in vivo obligate GroE substrates with the aid of data from an in vitro comprehensive analysis. The newly identified substrates have similar physicochemical properties to the known substrates, but their expression levels in vivo were significantly lower. Information from the in vitro comprehensive analysis has the potential to compensate for limitations of the MS-based proteomic approaches.

本文言語English
ページ(範囲)251-257
ページ数7
ジャーナルFEBS Letters
590
2
DOI
出版ステータスPublished - 2016 1月 1

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 細胞生物学
  • 遺伝学
  • 分子生物学
  • 構造生物学

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