Identification of proteasome components required for apical localization of chaoptin using functional genomics

Hiroyuki Yano, Miki Yamamoto-Hino, Wakae Awano, Kiyoko F. Aoki-Kinoshita, Kayoko Tsuda-Sakurai, Hideyuki Okano, Satoshi Goto

研究成果: Article

3 引用 (Scopus)

抄録

The distinct localization of membrane proteins with regard to cell polarity is crucial for the structure and function of various organs in multicellular organisms. However, the molecules and mechanisms that regulate protein localization to particular subcellular domains are still largely unknown. To identify the genes involved in regulation of protein localization, the authors performed a large-scale screen using a Drosophila RNA interference (RNAi) library, by which Drosophila genes could be knocked down in a tissue- and stage-specific manner. Drosophila photoreceptor cells have a morphologically distinct apicobasal polarity, along which Chaoptin (Chp), a glycosylphosphatidylinositol (GPI)-anchored membrane protein, and the Na +, K + -ATPase are localized to the apical and basolateral domains, respectively. By examining the subcellular localization of these proteins, the authors identified 106 genes whose knockdown resulted in mislocalization of Chp and Na +, K + -ATPase. Gene ontology analysis revealed that the knockdown of proteasome components resulted in mislocalization of Chp to the basolateral plasma membrane. These results suggest that the proteasome is involved, directly or indirectly, in selective localization of Chp to the apical plasma membrane of Drosophila photoreceptor cells.

元の言語English
ページ(範囲)53-63
ページ数11
ジャーナルJournal of Neurogenetics
26
発行部数1
DOI
出版物ステータスPublished - 2012 3

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Proteasome Endopeptidase Complex
Genomics
Drosophila
Photoreceptor Cells
Membrane Proteins
Cell Membrane
Gene Knockdown Techniques
Glycosylphosphatidylinositols
Cell Polarity
Gene Ontology
Proteins
RNA Interference
Genes
Libraries
sodium-translocating ATPase

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Genetics

これを引用

Identification of proteasome components required for apical localization of chaoptin using functional genomics. / Yano, Hiroyuki; Yamamoto-Hino, Miki; Awano, Wakae; Aoki-Kinoshita, Kiyoko F.; Tsuda-Sakurai, Kayoko; Okano, Hideyuki; Goto, Satoshi.

:: Journal of Neurogenetics, 巻 26, 番号 1, 03.2012, p. 53-63.

研究成果: Article

Yano, H, Yamamoto-Hino, M, Awano, W, Aoki-Kinoshita, KF, Tsuda-Sakurai, K, Okano, H & Goto, S 2012, 'Identification of proteasome components required for apical localization of chaoptin using functional genomics', Journal of Neurogenetics, 巻. 26, 番号 1, pp. 53-63. https://doi.org/10.3109/01677063.2012.661497
Yano, Hiroyuki ; Yamamoto-Hino, Miki ; Awano, Wakae ; Aoki-Kinoshita, Kiyoko F. ; Tsuda-Sakurai, Kayoko ; Okano, Hideyuki ; Goto, Satoshi. / Identification of proteasome components required for apical localization of chaoptin using functional genomics. :: Journal of Neurogenetics. 2012 ; 巻 26, 番号 1. pp. 53-63.
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