Identification of the sporulation gene spoOA product of Bacillus subtilis

Jun Kudoh, Toshihiko Ikeuchi, Kiyoshi Kurahashi

研究成果: Article査読

9 被引用数 (Scopus)

抄録

A 2.4-kilobase fragment of the Bacillus subtilis chromosome containing the wild-type spoOA gene derived from the φ105dspoOA+-Bc-1 transducing phage was cloned onto plasmid pBR322 in Escherichia coli. A recombinant plasmid harboring the mutant spoOA12 allele on the 2.4-kilobase insert was also constructed from the φ105dspoOA12-1 phage DNA and pBR322. Protein products synthesized in reponse to plasmid DNA in a DNA-directed cell-free system derived from E. coli were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A protein of approximately 27,500 daltons synthesized with the recombinant plasmid DNA harboring the wild-type spoOA gene as template was not formed with the recombinant plasmid DNA harboring the spoOA12 allele. Since the spoOA12 mutation is a nonsense mutation, we conclude that the 27.5-kilodalton protein is the product of the spoOA gene.

本文言語English
ページ(範囲)1104-1109
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
122
3
DOI
出版ステータスPublished - 1984 8 16

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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