Identification of TRP-2 as a human tumor antigen recognized by cytotoxic T lymphocytes

Rong Fu Wang, Ettore Appella, Yutaka Kawakami, Xiaoqiang Kang, Steven A. Rosenberg

研究成果: Article査読

271 被引用数 (Scopus)

抄録

The infusion of TIL586 along with interleukin-2 into the autologous patient with metastatic melanoma resulted in the objective regression of tumor. A gene encoding a tumor antigen recognized by TIL586 was previously isolated and shown to encode gp75 or TRP-1. Here we report that TRP-2 was identified as a second tumor antigen recognized by a HLA-A31-restricted CTL clone derived from the TIL586 cell line. The peptide LLPGGRPYR epitope was subsequently identified from the coding region of TRP-2 based on studies of the recognition of truncated TRP-2 cDNAs and the HLA-A31 binding motif. This epitope peptide was capable of sensitizing target cells for lysis by a CTL clone at 1 nM peptide concentration. Although some modified peptides could be recognized by the CTL clone, none were found to be better recognized by T cells than the parental peptide. Like other melanoma differentiation antigens, TRP-2 was only expressed in melanoma, melanocytes, and retina, but not in other human tissues tested.

本文言語English
ページ(範囲)2207-2216
ページ数10
ジャーナルJournal of Experimental Medicine
184
6
DOI
出版ステータスPublished - 1996 12 1
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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