Identification of two novel clusters of ultrahigh-sulfur keratin-associated protein genes on human chromosome 11

Shoichi Yahagi, Kazunori Shibuya, Izumi Obayashi, Hitoshi Masaki, Yoshiko Kurata, Jun Kudoh, Nobuyoshi Shimizu

研究成果: Article査読

24 被引用数 (Scopus)

抄録

We analyzed two novel clusters of keratin-associated protein (KAP) genes on human chromosome 11 (11p15.5 and 11q13.5) in which we identified two known human KRTAP5 genes, KerA (=KRN1) and KerB, and nine novel KRTAP5 family genes. RT-PCR analysis of these KAP genes showed preferential expression in human hair root, suggesting these gene products are required for hair formation. Based on the deduced amino acid sequences, all these KAP proteins were classified into an ultrahigh-sulfur (UHS) type KAP with high cysteine content (>30mol%). These KAPs also showed high glycine and serine contents (average 24.30 and 21.13mol%, respectively), distinguishing from other UHS/HS KAP families located on human chromosomes 17 and 21. Dot-matrix analysis revealed a significant similarity between these two KAP gene clusters. We postulated a mechanism by which these two KAP gene clusters are generated via genomic duplication of a primordial gene cluster followed by genetic modification during evolution.

本文言語English
ページ(範囲)655-664
ページ数10
ジャーナルBiochemical and Biophysical Research Communications
318
3
DOI
出版ステータスPublished - 2004 6月 4

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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