Identity of neocortical layer 4 neurons is specified through correct positioning into the cortex

Koji Oishi, Nao Nakagawa, Kashiko Tachikawa, Shinji Sasaki, Michihiko Aramaki, Shinji Hirano, Nobuhiko Yamamoto, Yumiko Yoshimura, Kazunori Nakajima

研究成果: Article

17 引用 (Scopus)

抜粋

Many cell-intrinsic mechanisms have been shown to regulate neuronal subtype specification in the mammalian neocortex. However, how much cell environment is crucial for subtype determination still remained unclear. Here, we show that knockdown of Protocadherin20 (Pcdh20), which is expressed in post-migratory neurons of layer 4 (L4) lineage, caused the cells to localize in L2/3. The ectopically positioned “future L4 neurons” lost their L4 characteristics but acquired L2/3 characteristics. Knockdown of a cytoskeletal protein in the future L4 neurons, which caused random disruption of positioning, also showed that those accidentally located in L4 acquired the L4 characteristics. Moreover, restoration of positioning of the Pcdh20-knockdown neurons into L4 rescued the specification failure. We further suggest that the thalamocortical axons provide a positional cue to specify L4 identity. These results suggest that the L4 identity is not completely determined at the time of birth but ensured by the surrounding environment after appropriate positioning.

元の言語English
記事番号e10907
ジャーナルeLife
5
発行部数FEBRUARY2016
DOI
出版物ステータスPublished - 2016 2 12

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

フィンガープリント Identity of neocortical layer 4 neurons is specified through correct positioning into the cortex' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Oishi, K., Nakagawa, N., Tachikawa, K., Sasaki, S., Aramaki, M., Hirano, S., Yamamoto, N., Yoshimura, Y., & Nakajima, K. (2016). Identity of neocortical layer 4 neurons is specified through correct positioning into the cortex. eLife, 5(FEBRUARY2016), [e10907]. https://doi.org/10.7554/eLife.10907