Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver

Norio Miyamura; Takashi Nakamura; Naoko Goto-Inoue; Nobuhiro Zaima; Takahiro Hayasaka; Tokiwa Yamasaki; Shuji Terai; Isao Sakaida; Mitsutoshi Setou; Hiroshi Nishina

doi:10.1016/j.bbrc.2011.03.133

Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver

Norio Miyamura, Takashi Nakamura, Naoko Goto-Inoue, Nobuhiro Zaima, Takahiro Hayasaka, Tokiwa Yamasaki, Shuji Terai, Isao Sakaida, Mitsutoshi Setou, Hiroshi Nishina

研究成果: Article › 査読

14 被引用数 (Scopus)

抄録

After partial hepatectomy (PH), regenerating liver accumulates unknown lipid species. Here, we analyzed lipids in murine liver and adipose tissues following PH by thin-layer chromatography (TLC), imaging mass spectrometry (IMS), and real-time RT-PCR. In liver, IMS revealed that a single TLC band comprised major 19 TG species. Similarly, IMS showed a single phospholipid TLC band to be major 13 species. In adipose tissues, PH induced changes to expression of genes regulating lipid metabolism. Finally, IMS of phosphatidylcholine species demonstrated distribution gradients in lobules that resembled hepatic zonation. IMS is thus a novel and power tool for analyzing lipid species with high resolution.

本文言語	English
ページ（範囲）	120-125
ページ数	6
ジャーナル	Biochemical and Biophysical Research Communications
巻	408
号	1
DOI	https://doi.org/10.1016/j.bbrc.2011.03.133
出版ステータス	Published - 2011 4月 29
外部発表	はい

ASJC Scopus subject areas

生物理学
生化学
分子生物学
細胞生物学

文献へのアクセス

10.1016/j.bbrc.2011.03.133

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引用スタイル

Miyamura, N., Nakamura, T., Goto-Inoue, N., Zaima, N., Hayasaka, T., Yamasaki, T., Terai, S., Sakaida, I., Setou, M., & Nishina, H. (2011). Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver. Biochemical and Biophysical Research Communications, 408(1), 120-125. https://doi.org/10.1016/j.bbrc.2011.03.133

Miyamura, N, Nakamura, T, Goto-Inoue, N, Zaima, N, Hayasaka, T, Yamasaki, T, Terai, S, Sakaida, I, Setou, M & Nishina, H 2011, 'Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver', Biochemical and Biophysical Research Communications, vol. 408, no. 1, pp. 120-125. https://doi.org/10.1016/j.bbrc.2011.03.133

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title = "Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver",

abstract = "After partial hepatectomy (PH), regenerating liver accumulates unknown lipid species. Here, we analyzed lipids in murine liver and adipose tissues following PH by thin-layer chromatography (TLC), imaging mass spectrometry (IMS), and real-time RT-PCR. In liver, IMS revealed that a single TLC band comprised major 19 TG species. Similarly, IMS showed a single phospholipid TLC band to be major 13 species. In adipose tissues, PH induced changes to expression of genes regulating lipid metabolism. Finally, IMS of phosphatidylcholine species demonstrated distribution gradients in lobules that resembled hepatic zonation. IMS is thus a novel and power tool for analyzing lipid species with high resolution.",

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author = "Norio Miyamura and Takashi Nakamura and Naoko Goto-Inoue and Nobuhiro Zaima and Takahiro Hayasaka and Tokiwa Yamasaki and Shuji Terai and Isao Sakaida and Mitsutoshi Setou and Hiroshi Nishina",

note = "Funding Information: This work was supported in part by research grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan , the Ministry of Health, Labour and Welfare of Japan , and the Japan Society for the Promotion of Science . ",

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AU - Hayasaka, Takahiro

AU - Yamasaki, Tokiwa

AU - Terai, Shuji

AU - Sakaida, Isao

AU - Setou, Mitsutoshi

AU - Nishina, Hiroshi

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