Immediate electrical physiological damage dependence of myocardial cells on irradiance and photosensitizer concentration

Emiyu Ogawa, Tsunenori Arai

研究成果: Article査読

抄録

We studied the dependence of immediate myocardial cell response on irradiance and photosensitizer concentration by an extracellular photosensitization reaction. We have proposed a new methodology for tachyarrhythmia treatment using the extracellular photosensitization reaction. Immediate cell response is needed in the tachyarrhythmia treatment since electrical physiological diagnosis is performed during the operation to judge the treatment effect. Intracellular Ca<sup>2+</sup> during and after the reaction was measured using a confocal microscope and Fluo-4 AM as Ca<sup>2+</sup> dye. The photosensitizer concentration, irradiance, and radiant exposure were varied 10-30 μg/ml, 30-293 mW/cm<sup>2</sup>, and 10-40 J/cm<sup>2</sup>, respectively. We found that the necrosis occurrence timing after the laser irradiation beginning was decreased with the photosensitizer concentration and irradiance increasing. The minimum necrosis occurrence timing was 209 s with 30 μg/ml in photosensitizer concentration and 293 mW/cm<sup>2</sup> in irradiance.

本文言語English
ページ(範囲)O-70-O-71
ジャーナルTransactions of Japanese Society for Medical and Biological Engineering
52
DOI
出版ステータスPublished - 2014 8月 17

ASJC Scopus subject areas

  • 生体医工学

フィンガープリント

「Immediate electrical physiological damage dependence of myocardial cells on irradiance and photosensitizer concentration」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル