Immediate response of Ca2+ concentration in myocardial cells against oxidation stress by extracellular photosensitization reaction using Talaporfin sodium for the arrhythmia treatment application

Emiyu Ogawa, Mei Takahashi, Arisa Ito, Tsunenori Arai

研究成果: Conference contribution

4 被引用数 (Scopus)

抄録

We studied the immediate response of myocardial cells by continuous observation using confocal microscope against oxidation stress by extracellular photosensitization reaction using Talaporfin sodium for tachyarrhythmia treatment application. Immediate response in order from several seconds to several minutes is required for the arrhythmia treatment since operators should judge the therapeutic effect during the tachyarrhythmia ablation procedure. To understand the immediate response of myocardial cells, we measured the intracellular Ca2+ concentration using fluo-4 AM during and after the extracellular photosensitization reaction. Talaporfin sodium concentration was varied 10-30 μg/ml. A red diode laser of 663 nm in wavelength was irradiated under the microscope with the radiant exposure of 40 J/cm2 and irradiance of 0.29 W/cm2. We observed the fluorescence image of fluo-4 AM each 400 ms during until 10 min after the photosensitization reaction. The myocardial cell beatings were stopped about 2 s after the beginning of the laser irradiation. The blebs were formed with the Ca2+ inflow. The intracellular Ca2+ was re-decreased after the bleb formation and then the cell necrosis was induced. The cell lethality 10 min after the laser irradiation was less than bleb formation ratio. The time response of the cell necrosis was shortened with the photosensitizer concentration increasing and the minimum average value was 209 s in the case of the 30 μg/ml in photosensitizer concentration and 40 J/cm2 in the radiant exposure. We think this extracellular photosensitization reaction may be applicable to tachyarrhythmia treatment in terms of its immediate response.

本文言語English
ホスト出版物のタイトルOptical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications
出版社SPIE
ISBN(印刷版)9780819498540
DOI
出版ステータスPublished - 2014
イベントOptical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications - San Francisco, CA, United States
継続期間: 2014 2 22014 2 4

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
8941
ISSN(印刷版)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications
CountryUnited States
CitySan Francisco, CA
Period14/2/214/2/4

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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