Immune suppressor factor confers stromal cell line with enhanced supporting activity for hematopoietic stem cells

Hideaki Nakajima, Fumi Shibata, Yumi Fukuchi, Yuko Goto-Koshino, Miyuki Ito, Atsushi Urano, Tatsutoshi Nakahata, Hiroyuki Aburatani, Toshio Kitamura

研究成果: Article査読

8 被引用数 (Scopus)

抄録

Immune suppressor factor (ISF) is a subunit of the vacuolar ATPase proton pump. We earlier identified a short form of ISF (ShIF) as a stroma-derived factor that supports cytokine-independent growth of mutant Ba/F3 cells. Here, we report that ISF/ShIF supports self-renewal and expansion of primary hematopoietic stem cells (HSCs). Co-culture of murine bone marrow cells with a stromal cell line overexpressing ISF or ShIF (MS10/ISF or MS10/ShIF) not only enhanced their colony-forming activity and the numbers of long-term culture initiating cells, but also maintained the competitive repopulating activity of HSC. This stem cell supporting activity depended on the proton-transfer function of ISF/ShIF. Gene expression analysis of ISF/ShIF-transfected cell lines revealed down-regulation of secreted frizzled-related protein-1 and tissue inhibitor of metalloproteinase-3, and the restoration of their expressions in MS10/ISF cells partially reversed its enhanced LTC-IC supporting activity to a normal level. These results suggest that ISF/ShIF confers stromal cells with enhanced supporting activities for HSCs by modulating Wnt-activity and the extracellular matrix.

本文言語English
ページ(範囲)35-42
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
340
1
DOI
出版ステータスPublished - 2006 2月 3
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Immune suppressor factor confers stromal cell line with enhanced supporting activity for hematopoietic stem cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル