@article{3b1ed1cf92eb40de85b8f726c2e8a43a,
title = "Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing piRNA biogenesis bodies in Drosophila ovaries",
abstract = "Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specifi c proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specifi c target labeled with both fl uorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.",
keywords = "EM-ISH, Electron microscopic in situ hybridization, Electron microscopy, Flam, Fluorescence immunohistochemistry, ISH, Immuno-electron microscopy, In situ hybridization, PIWI-interacting RNAs, PiRNAs, Yb, Zuc",
author = "Shinsuke Shibata and Yukiko Murota and Yoshinori Nishimoto and Mana Yoshimura and Toshihiro Nagai and Hideyuki Okano and Siomi, {Mikiko C.}",
note = "Publisher Copyright: {\textcopyright} 2015, Springer Science+Business Media New York.",
year = "2015",
doi = "10.1007/978-1-4939-2851-4_12",
language = "English",
volume = "1328",
pages = "163--178",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",
}