Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing piRNA biogenesis bodies in Drosophila ovaries

Shinsuke Shibata, Yukiko Murota, Yoshinori Nishimoto, Mana Yoshimura, Toshihiro Nagai, Hideyuki Okano, Mikiko C. Siomi

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specifi c proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specifi c target labeled with both fl uorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.

本文言語English
ページ(範囲)163-178
ページ数16
ジャーナルMethods in Molecular Biology
1328
DOI
出版ステータスPublished - 2015

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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