Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing pirna biogenesis bodies in Drosophila ovaries

Shinsuke Shibata, Yukiko Murota, Yoshinori Nishimoto, Mana Yoshimura, Toshihiro Nagai, Hideyuki Okano, Mikiko C. Siomi

研究成果: Chapter

1 被引用数 (Scopus)

抄録

Immuno-electron microscopy and electron microscopic in situ hybridization are powerful tools to identify the precise subcellular localization of specific proteins and RNAs at the ultramicroscopic level. Here we describe detailed procedures for how to detect the precise location of a specific target labeled with both fluorescence and gold particles. Although they have been developed for the analysis of Drosophila ovarian somatic cells, these techniques are suitable for a wide range of biological applications including human, primate, and rodent analysis.

本文言語English
ホスト出版物のタイトルDrosophila Oogenesis
ホスト出版物のサブタイトルMethods and Protocols
出版社Springer New York
ページ163-178
ページ数16
ISBN(電子版)9781493928514
ISBN(印刷版)9781493928507
DOI
出版ステータスPublished - 2015 8月 31

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 医学(全般)

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