抄録
NOD/Shi-scid IL-2Rγnull (NOG) mice established by introducing the IL-2Rγnull gene of IL-2Rγ KO mice into NOD/Shi-scid mice by backcross-mating show a high xenograft engraftment level and are therefore well suited as a humanized mouse model. SCID mice bearing the Prkdcscid gene show a high incidence of thymic lymphoma and a leaky phenomenon in which a few clonal T and B cells develop in aged mice. In the present study, NOG mice were assessed for the presence of a leaky phenomenon such as the one observed in C.B-17-scid and NOD-scid mice. Serum immunoglobulin analysis did not detect IgG or IgM in NOG mice, unlike the findings in C.B-17-scid and NOD-scid mice. Flow cytometry analysis revealed the absence of T and B cells in the peripheral blood and spleens of NOG mice. These results reflect the suppression of the leaky phenomenon in NOG mice through the inactivation of the IL-2Rγ gene, which is commonly expressed in T and B cell growth factor receptors to IL-2, IL-4 and IL-7.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 181-186 |
| ページ数 | 6 |
| ジャーナル | Experimental Animals |
| 巻 | 60 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | Published - 2011 |
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ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
- Biochemistry, Genetics and Molecular Biology(all)
これを引用
Immunodeficient NOD-scid IL-2Rγnull mice do not display T and B cell leakiness. / Katano, Ikumi; ITO, Ryoji; Eto, Tomoo; Aiso, Sadakazu; Ito, Mamoru.
：: Experimental Animals, 巻 60, 番号 2, 2011, p. 181-186.研究成果: Article
}
TY - JOUR
T1 - Immunodeficient NOD-scid IL-2Rγnull mice do not display T and B cell leakiness
AU - Katano, Ikumi
AU - ITO, Ryoji
AU - Eto, Tomoo
AU - Aiso, Sadakazu
AU - Ito, Mamoru
PY - 2011
Y1 - 2011
N2 - NOD/Shi-scid IL-2Rγnull (NOG) mice established by introducing the IL-2Rγnull gene of IL-2Rγ KO mice into NOD/Shi-scid mice by backcross-mating show a high xenograft engraftment level and are therefore well suited as a humanized mouse model. SCID mice bearing the Prkdcscid gene show a high incidence of thymic lymphoma and a leaky phenomenon in which a few clonal T and B cells develop in aged mice. In the present study, NOG mice were assessed for the presence of a leaky phenomenon such as the one observed in C.B-17-scid and NOD-scid mice. Serum immunoglobulin analysis did not detect IgG or IgM in NOG mice, unlike the findings in C.B-17-scid and NOD-scid mice. Flow cytometry analysis revealed the absence of T and B cells in the peripheral blood and spleens of NOG mice. These results reflect the suppression of the leaky phenomenon in NOG mice through the inactivation of the IL-2Rγ gene, which is commonly expressed in T and B cell growth factor receptors to IL-2, IL-4 and IL-7.
AB - NOD/Shi-scid IL-2Rγnull (NOG) mice established by introducing the IL-2Rγnull gene of IL-2Rγ KO mice into NOD/Shi-scid mice by backcross-mating show a high xenograft engraftment level and are therefore well suited as a humanized mouse model. SCID mice bearing the Prkdcscid gene show a high incidence of thymic lymphoma and a leaky phenomenon in which a few clonal T and B cells develop in aged mice. In the present study, NOG mice were assessed for the presence of a leaky phenomenon such as the one observed in C.B-17-scid and NOD-scid mice. Serum immunoglobulin analysis did not detect IgG or IgM in NOG mice, unlike the findings in C.B-17-scid and NOD-scid mice. Flow cytometry analysis revealed the absence of T and B cells in the peripheral blood and spleens of NOG mice. These results reflect the suppression of the leaky phenomenon in NOG mice through the inactivation of the IL-2Rγ gene, which is commonly expressed in T and B cell growth factor receptors to IL-2, IL-4 and IL-7.
KW - Immunoglobulin
KW - Leakiness
KW - NOD-scid il-2Rγnull mice
UR - http://www.scopus.com/inward/record.url?scp=79955420739&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955420739&partnerID=8YFLogxK
U2 - 10.1538/expanim.60.181
DO - 10.1538/expanim.60.181
M3 - Article
C2 - 21512274
AN - SCOPUS:79955420739
VL - 60
SP - 181
EP - 186
JO - Experimental Animals
JF - Experimental Animals
SN - 1341-1357
IS - 2
ER -