Immunohistochemical detection of K-sam protein in stomach cancer

Yutaka Hattori, Hiroshi Itoh, Shinya Uchino, Kouichi Hosokawa, Atsushi Ochiai, Yoshinori Ino, Hideshi Ishii, Hiromi Sakamoto, Naohito Yamaguchi, Kazuyoshi Yanagihara, Setsuo Hirohashi, Takashi Sugimura, Masaaki Terada

研究成果: Article査読

116 被引用数 (Scopus)


The K-sam gene, originally isolated as an amplified gene from the stomach cancer cell line KATO-III, is characterized by its preferential amplification in the undifferentiated type (diffuse type) of stomach cancer and encodes one of the receptors for heparin-binding growth factors or fibroblast growth factors. The K-sam gene has been isolated by different methods and has been designated BEK, TK14, and Cek2. The receptor for keratinocyte growth factor was also found to be encoded by the same gene. To examine the expression of the K-sam protein in stomach cancer, polyclonal antibody pK1-2 was raised against the extracellular domain of the gene product. This antibody detected K-sam proteins by Western blot and flow cytometry analyses in stomach cancer cell lines KATO-III and HSC39, in which the K-sam gene is amplified and overexpressed. By immunohistochemical analysis, 20 of 38 cases of the undifferentiated type of advanced stomach cancer were K-sam positive, whereas none of 11 cases of the differentiated or intestinal type revealed K-sam staining. The K-sam product was observed predominantly in diffusely infiltrative lesions. In one autopsy case, the K- sam protein was detected only locally in the primary tumor, whereas markedly increased staining for the K-sam product was detected diffusely in the metastasized tumor in the lymph node and liver. These results suggest that K- sam overexpression is associated with the malignant phenotype of the undifferentiated type of stomach cancer, such as infiltrative growth and metastasis.

ジャーナルClinical Cancer Research
出版ステータスPublished - 1996 8 1

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究


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