TY - JOUR
T1 - Immunohistochemical studies on mouse cerebral cortex grafted into the third ventricle of rats treated with cyclosporin A
AU - Inoue, Hiroshi
AU - Kohsaka, Shinichi
AU - Yoshida, Kazunari
AU - Otani, Mitsuhiro
AU - Toya, Shigeo
AU - Tsukada, Yasuzo
N1 - Funding Information:
Cyclosporin A was supplied by Sandoz Ltd. (Switzerland). This work was partially supported by grants from Keio University and the Japan Brain Foundation.
PY - 1985/6/24
Y1 - 1985/6/24
N2 - Newborn mouse cerebral cortex was transplanted into the third ventricle of an adult rat brain. After the operation, the immunosuppressant cyclosporin A was administered daily for two weeks, and the grafted tissue was examined immunohistochemically at the 4th week. Neurons, astroglias and oligoden-droglias were positively stained by antibodies against neurofilament, glial fibrillary acid protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), respectively. The CNPase staining indicated that myelination had occurred in the grafted tissue. Thus both neuronal and glial elements in the grafted tissue could survive and mature in the third ventricle of the different species upon administration of cyclosporin A.
AB - Newborn mouse cerebral cortex was transplanted into the third ventricle of an adult rat brain. After the operation, the immunosuppressant cyclosporin A was administered daily for two weeks, and the grafted tissue was examined immunohistochemically at the 4th week. Neurons, astroglias and oligoden-droglias were positively stained by antibodies against neurofilament, glial fibrillary acid protein and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), respectively. The CNPase staining indicated that myelination had occurred in the grafted tissue. Thus both neuronal and glial elements in the grafted tissue could survive and mature in the third ventricle of the different species upon administration of cyclosporin A.
KW - 2',3'-cyclic nucleotide 3'-phosphodiesterase
KW - CNPase
KW - GFAP
KW - cyclosporin A
KW - glial fibrillary acidic protein
KW - immunohistochemistry
KW - myelination
KW - neural transplantation
KW - neurofilament
KW - rat
UR - http://www.scopus.com/inward/record.url?scp=0021819742&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021819742&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(85)90507-5
DO - 10.1016/0304-3940(85)90507-5
M3 - Article
C2 - 4034098
AN - SCOPUS:0021819742
VL - 57
SP - 289
EP - 294
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 3
ER -