Immunohistochemical study of. GAMMA.-glutamyl transpeptidase with monoclonal antibodies. I Preparation and characteristics of monoclonal antibodies to. GAMMA.-glutamyl transpeptidase

Kenjiro Yasuda, Yamashita Shuji, Sadakazu Aiso, Masahide Shiozawa, Toshiko Komatsu

研究成果: Article査読

29 被引用数 (Scopus)

抄録

Monoclonal antibodies of the IgG1 subclass were prepared against rat kidney γ-glutamyl transpeptidase by the hybridoma technique. Eight hybrid clones produced antibodies against the enzyme. Finally two stable clones which produce specific antibodies were obtained. The antibodies specifically recognized a heavy subunit (Mr=51, 000) of the enzyme. Immunohistochemical examination revealed that the reaction was localized in the brush border as well as along the basolateral membrane of the proximal convolution of rat kidney and also in the epididymis and the pancreas of rats. Human kidney showed positive reaction, whereas no reaction was recognized in the kidneys of other species, such as mice, guinea pigs and rabbits.

本文言語English
ページ(範囲)589-600
ページ数12
ジャーナルActa Histochemica et Cytochemica
19
5
DOI
出版ステータスPublished - 1986

ASJC Scopus subject areas

  • 病理学および法医学
  • 生化学
  • 生理学
  • 組織学
  • 細胞生物学

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