In situ cancer vaccination with a replication-conditional HSV for the treatment of liver metastasis of colon cancer

Takashi Endo, Masahiro Toda, Masahiko Watanabe, Yukihiko Iizuka, Tetsuro Kubota, Masaki Kitajima, Yutaka Kawakami

研究成果: Article査読

47 被引用数 (Scopus)

抄録

In this study, we investigated the therapeutic efficacy of a replication-conditional mutant HSV, G207, for the treatment of liver metastasis of colon carcinoma. Three liver metastasis models in syngeneic BALB/c mice were developed: (i) splenic injection, (ii) splenic and subcutaneous (s.c.) injection, and (iii) orthotopic implantation of CT26 colon carcinoma. In the splenic injection model, G207 was injected into the established splenic tumor on day 7. In the splenic and s.c. injection model, G207 were injected into the established s.c. tumor on days 5 and 8. In the orthotopic implantation model, a piece of CT26 tumor tissue was transplanted onto the wall of the cecum and G207 was injected in the established cecum tumor on day 7. On day 21 or 28, animals were sacrificed and liver metastases were evaluated. In all three models in immunocompetent mice, liver metastases were significantly reduced by intratumoral inoculation with G207 compared to the control. In athymic mice, however, there was no significant therapeutic effect of intratumoral inoculation with G207 on liver metastases. Tumor-specific cytotoxic T-lymphocyte responses were induced in mice treated with G207 in the orthotopic implantation model. These results suggest that intratumoral inoculation of G207, as an in situ cancer vaccine, can be an effective approach against liver metastasis of colon cancer and the efficacy involves tumor-specific T-cell responses.

本文言語English
ページ(範囲)142-148
ページ数7
ジャーナルCancer Gene Therapy
9
2
DOI
出版ステータスPublished - 2002

ASJC Scopus subject areas

  • 分子医療
  • 分子生物学
  • 癌研究

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