In vitro anti-DNA antibody synthesis by peripheral blood lymphocytes in patients with systemic lupus erythematosus

Masaya Kiyotaki, Takashi Toguchi, Tohru Abe, Tsutomu Takeuchi, Chikao Morimoto, Mitsuo Homma

研究成果: Article査読

12 被引用数 (Scopus)

抄録

In vitro anti-DNA antibody synthesis by peripheral blood lymphocytes from systemic lupus erythematosus (SLE) patients after native DNA (nDNA) stimulation was studied by the Farr assay after digestion with deoxyribonuclease I. Active SLE lymphocytes synthetized IgG and anti-DNA antibody with nDNA stimulation but inactive SLE and normal lymphocytes did not. Binding of 14C-labeled Ig and [3H]DNA showed that DNA-binding Ig was actively secreted by SLE lymphocytes. SLE antilymphocyte antibody (ALA)-sensitive T cells (SLE+ T cells) and unreactive T cells (SLE- T cells) were separated by anti-human Ig antibody-coated dishes to determine their effect on antibody synthesis. Inactive SLE B cells synthetized both IgG and anti-DNA antibody after nDNA stimulation with autologous SLE- T cells but stimulation with SLE+ T cells did not result in synthesis of either. Normal B cells did not synthetize IgG even with autologous SLE- T cells. These results indicate that active and inactive SLE patients have autoreactive lymphocytes against nDNA which synthetize anti-DNA antibody and are regulated by ALA-sensitive T cells (possibly deficient in active SLE).

本文言語English
ページ(範囲)237-246
ページ数10
ジャーナルClinical Immunology and Immunopathology
21
2
DOI
出版ステータスPublished - 1981 11

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 病理学および法医学
  • 免疫学

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