In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells

Taisuke Kondo, Yuki Imura, Makoto Ando, Shunsuke Chikuma, Akihiko Yoshimura

研究成果: Chapter

3 被引用数 (Scopus)

抄録

Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ41-51
ページ数11
DOI
出版ステータスPublished - 2019

出版物シリーズ

名前Methods in Molecular Biology
2048
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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