In vitro effects of angiopoietins and VEGF on hematopoietic and endothelial cells

Xu Ling Huang, Nobuyuki Takakura, Toshio Suda

研究成果: Article査読

28 被引用数 (Scopus)

抄録

To investigate the role of TIE-2/angiopoietins (Angs) in postnatal hematopoiesis, we cultured bone marrow cells in the presence of Ang-1 and -2 with or without VEGF. While either Ang-1 or Ang-2 alone had hematopoietic effects on unseparated bone marrow cells and no effect on proliferation of endothelial cells, both enhanced the growth of endothelial cells and hematopoietic progenitor cells in the presence of VEGF. FACS analysis showed that Lin-TIE-2+Flk-1+ cells cocultured with OP9 stromal cells gave rise to endothelial and hematopoietic cells in the presence of VEGF and Ang-1. Ang-1 promoted the adhesion of sorted primary Lin-TIE-2+ cells to fibronectin, and this adhesion enhanced proliferation of hematopoietic progenitor cells synergistically with stem cell factor (SCF). Our findings suggest that TIE-2/angiopoietins may act as critical regulators of proliferation of hematopoietic progenitors and endothelial cells in a synergistic manner.

本文言語English
ページ(範囲)133-138
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
264
1
DOI
出版ステータスPublished - 1999 10 14

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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