In vitro free radical scavenging activity of platinum nanoparticles

Aki Watanabe, Masashi Kajita, Juewon Kim, Atsuhiro Kanayama, Kyoko Takahashi, Tadahiko Mashino, Yusei Miyamoto

研究成果: Article査読

145 被引用数 (Scopus)


A polyacrylic acid (PAA)-protected platinum nanoparticle species (PAA-Pt) was prepared by alcohol reduction of hexachloroplatinate. The PAA-Pt nanoparticles were well dispersed and homogeneous in size with an average diameter of 2.0 0.4nm (n = 200). We used electron spin resonance to quantify the residual peroxyl radical () generated from 2,2-azobis (2-aminopropane) dihydrochloride (AAPH) by thermal decomposition in the presence of O2 and a spectrophotometric method to quantify the residual 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical. PAA-Pt scavenged these two radicals in a dose-dependent manner. Platinum was the functional component. PAA-Pt reduced the rate of oxygen consumption required for linoleic acid peroxidation initiated by generated from AAPH, indicating inhibition of the propagation of linolate peroxidation. A thiobarbituric acid test also revealed dose-dependent inhibition of the linolate peroxidation by PAA-Pt. Fifty micromolar platinum, as PAA-Pt, completely quenched 250 νM DPPH radical for 5min. Even when twice diluted in half, the PAA-Pt still quenched 100% of the 250νM DPPH radical. The scavenging activity of PAA-Pt is durable. These observations suggest that PAA-Pt is an efficient scavenger of free radicals.

出版ステータスPublished - 2009 11月 9

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 化学 (全般)
  • 材料科学(全般)
  • 材料力学
  • 機械工学
  • 電子工学および電気工学


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