In vitro gene transfer by the use of laser-induced stress wave: Effect of laser irradiation conditions on transfection efficiency

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

    研究成果: Conference article査読

    抄録

    Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm2, but at higher fluences (>1.7 J/cm2), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.

    本文言語English
    論文番号36
    ページ(範囲)225-229
    ページ数5
    ジャーナルProgress in Biomedical Optics and Imaging - Proceedings of SPIE
    5695
    DOI
    出版ステータスPublished - 2005 8 16
    イベントOptical Interactions with Tissue and Cells XVI - San Jose, CA, United States
    継続期間: 2005 1 242005 1 26

    ASJC Scopus subject areas

    • 電子材料、光学材料、および磁性材料
    • 生体材料
    • 原子分子物理学および光学
    • 放射線学、核医学およびイメージング

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