Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm2, but at higher fluences (>1.7 J/cm2), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.
|ジャーナル||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|出版ステータス||Published - 2005 8月 16|
|イベント||Optical Interactions with Tissue and Cells XVI - San Jose, CA, United States|
継続期間: 2005 1月 24 → 2005 1月 26
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