In vitro growth profiles of respiratory syncytial virus in the presence of influenza virus

Masayoshi Shinjo(H), K. Omoe, N. Saito, N. Matsuo, K. Nerome

研究成果: Article

23 引用 (Scopus)

抄録

To elucidate epidemiological interference between respiratory syncytial (RSV) and influenza viruses, the influence of influenza A (H1N1) virus on the growth of RSV was examined. Although RSV grew in MDCK cells, coinfection with influenza A virus led to a reduction of progeny RSV. The degree of growth interference depended on the time of infection with influenza A virus post infection (p.i.) with RSV. In fact, infection with influenza A virus 12 hrs p.i. with RSV did not influence growth of the latter virus. On the contrary, growth suppression of influenza A virus by RSV was observed when the coinfection began at the later stages of RSV infection. Suppression of the growth of RSV by influenza A infection was further demonstrated at the level of viral protein synthesis. An indirect immunofluorescence (IF) test revealed that a large proportion of infected cells synthesized both RSV and influenza A Virus antigens. Scanning electron microscopic (SEM) examination demonstrated that influenza A and RSV virions possessing surface antigens specific for each virus were selectively released from dually infected cells. In the present study, we proved for the first time that the growth of RSV is blocked by competitive infection with influenza A Virus in a susceptible cell population, competitive protein synthesis and selective budding of RSV and influenza Viruses from the same infected cells.

元の言語English
ページ(範囲)91-97
ページ数7
ジャーナルActa Virologica
44
発行部数2
出版物ステータスPublished - 2000

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Respiratory Syncytial Viruses
Influenza A virus
Orthomyxoviridae
Growth
Infection
Coinfection
Human Influenza
Viruses
H1N1 Subtype Influenza A Virus
Madin Darby Canine Kidney Cells
Viral Proteins
Virus Diseases
Surface Antigens
Indirect Fluorescent Antibody Technique
In Vitro Techniques
Virion
Electrons
Antigens
Population

ASJC Scopus subject areas

  • Virology
  • Immunology

これを引用

In vitro growth profiles of respiratory syncytial virus in the presence of influenza virus. / Shinjo(H), Masayoshi; Omoe, K.; Saito, N.; Matsuo, N.; Nerome, K.

:: Acta Virologica, 巻 44, 番号 2, 2000, p. 91-97.

研究成果: Article

Shinjo(H), M, Omoe, K, Saito, N, Matsuo, N & Nerome, K 2000, 'In vitro growth profiles of respiratory syncytial virus in the presence of influenza virus', Acta Virologica, 巻. 44, 番号 2, pp. 91-97.
Shinjo(H), Masayoshi ; Omoe, K. ; Saito, N. ; Matsuo, N. ; Nerome, K. / In vitro growth profiles of respiratory syncytial virus in the presence of influenza virus. :: Acta Virologica. 2000 ; 巻 44, 番号 2. pp. 91-97.
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