In vitro neurogenesis by progenitor cells isolated from the adult human hippocampus

Neeta Singh Roy, Su Wang, Li Jiang, Jian Kang, Abdellatif Benraiss, Catherine Harrison-Restelli, Richard A.R. Fraser, William T. Couldwell, Ayano Kawaguchi, Hideyuki Okano, Maiken Nedergaard, Steven A. Goldman

研究成果: Article査読

483 被引用数 (Scopus)

抄録

Neurogenesis persists in the adult mammalian hippocampus. To identify and isolate neuronal progenitor cells of the adult human hippocampus, we transfected ventricular zone-free dissociates of surgically-excised dentate gyrus with DNA encoding humanized green fluorescent protein (hGFP), placed under the control of either the nestin enhancer (E/nestin) or the Tα1 tubulin promoter (P/Tα1), two regulatory regions that direct transcription in neural progenitor cells. The resultant P/Tα1:hGFP+, and E/nestin:enhanced (E)GFP+ cells expressed βIII-tubulin or microtubule- associated protein-2; many incorporated bromodeoxyuridine, indicating their genesis in vitro. Using fluorescence-activated cell sorting, the E/nestin:EGFP+ and P/Tα1:hGFP+ cells were isolated to near purity, and matured antigenically and physiologically as neurons. Thus, the adult human hippocampus contains mitotically competent neuronal progenitors that can be selectively extracted. The isolation of these cells may provide a cellular substrate for re-populating the damaged or degenerated adult hippocampus.

本文言語English
ページ(範囲)271-277
ページ数7
ジャーナルNature medicine
6
3
DOI
出版ステータスPublished - 2000 3月
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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