In vitro neurogenesis by progenitor cells isolated from the adult human hippocampus

Neeta Singh Roy, Su Wang, Li Jiang, Jian Kang, Abdellatif Benraiss, Catherine Harrison-Restelli, Richard A.R. Fraser, William T. Couldwell, Ayano Kawaguchi, Hideyuki Okano, Maiken Nedergaard, Steven A. Goldman

研究成果: Article

457 引用 (Scopus)

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Neurogenesis persists in the adult mammalian hippocampus. To identify and isolate neuronal progenitor cells of the adult human hippocampus, we transfected ventricular zone-free dissociates of surgically-excised dentate gyrus with DNA encoding humanized green fluorescent protein (hGFP), placed under the control of either the nestin enhancer (E/nestin) or the Tα1 tubulin promoter (P/Tα1), two regulatory regions that direct transcription in neural progenitor cells. The resultant P/Tα1:hGFP+, and E/nestin:enhanced (E)GFP+ cells expressed βIII-tubulin or microtubule- associated protein-2; many incorporated bromodeoxyuridine, indicating their genesis in vitro. Using fluorescence-activated cell sorting, the E/nestin:EGFP+ and P/Tα1:hGFP+ cells were isolated to near purity, and matured antigenically and physiologically as neurons. Thus, the adult human hippocampus contains mitotically competent neuronal progenitors that can be selectively extracted. The isolation of these cells may provide a cellular substrate for re-populating the damaged or degenerated adult hippocampus.

元の言語English
ページ(範囲)271-277
ページ数7
ジャーナルNature medicine
6
発行部数3
DOI
出版物ステータスPublished - 2000 3 1
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Roy, N. S., Wang, S., Jiang, L., Kang, J., Benraiss, A., Harrison-Restelli, C., Fraser, R. A. R., Couldwell, W. T., Kawaguchi, A., Okano, H., Nedergaard, M., & Goldman, S. A. (2000). In vitro neurogenesis by progenitor cells isolated from the adult human hippocampus. Nature medicine, 6(3), 271-277. https://doi.org/10.1038/73119