TY - JOUR
T1 - In Vitro Osteogenic Activity of Rat Bone Marrow Derived Mesenchymal Stem Cells Cultured on Transparent Hydroxyapatite Ceramics
AU - Kotobuki, Noriko
AU - Kawagoe, Daisuke
AU - Fujimori, Hirotaka
AU - Goto, Seishi
AU - Loku, Koji
AU - Ohgushi, Hajime
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Direct observation of cultured cells on various materials has benefit for assessment of fundamental cellular functions including cell attachment, spreading, proliferation and differentiation on the materials. For this purpose, we made transparent hydroxyapatite (tHA) ceramics, where cultured rat bone marrow-derived mesenchymal stem cells (rMSCs) could be visible by phase contrast microscopy immediately after plating. The seeded rMSCs adhered and proliferated on the tHA ceramics. Furtheremore, the cells cultured on the tHA ceramics were able to differentiate into osteoblasts under osteogenic conditions and showed comparable osteogenic activity to the cells cultured on tissue culture polystyrene dishes. The results suggested that cultured cells could be monitored in a time dependent manner using the tHA ceramics.
AB - Direct observation of cultured cells on various materials has benefit for assessment of fundamental cellular functions including cell attachment, spreading, proliferation and differentiation on the materials. For this purpose, we made transparent hydroxyapatite (tHA) ceramics, where cultured rat bone marrow-derived mesenchymal stem cells (rMSCs) could be visible by phase contrast microscopy immediately after plating. The seeded rMSCs adhered and proliferated on the tHA ceramics. Furtheremore, the cells cultured on the tHA ceramics were able to differentiate into osteoblasts under osteogenic conditions and showed comparable osteogenic activity to the cells cultured on tissue culture polystyrene dishes. The results suggested that cultured cells could be monitored in a time dependent manner using the tHA ceramics.
KW - Mesenchymal stem cell
KW - Rat bone marrow
KW - Transparent hydroxyapatite ceramic
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M3 - Conference article
AN - SCOPUS:0347516082
VL - 254-256
SP - 1055
EP - 1058
JO - Key Engineering Materials
JF - Key Engineering Materials
SN - 1013-9826
T2 - The Annual Meeting of the International Society for Ceramics in Medicine
Y2 - 6 November 2003 through 9 November 2003
ER -