IN vitro production of autoantibodies to u1 ribonucleoproteins by peripheral blood mononuclear cells from patients with connective tissue diseases

Yasuo Ohosone, Masashi Akizuki, Michito Hirakata, Minoru Satoh, Hajime Yamagata, Mitsuo Homma

研究成果: Article

7 引用 (Scopus)

抜粋

Antibodies to U1 ribonucleoproteins produced by peripheral blood mononuclear cells from patients with connective tissue diseases were measured by a sensitive enzyme‐linked immunosorbent assay and by immunoblotting. Mononuclear cells from patients with high‐titer serum antibodies spontaneously started secreting IgG anti—U1 RNP antibodies on the second day after culture. The amount of anti—U1 RNP in the culture supernatants reached maximum level on day 8. The most effective production of anti—U1 RNP by B cells was observed when they were cultured in the presence of T cells and adherent cells. Mononuclear cells from patients without anti—U1 RNP antibodies or from normal subjects did not produce a measurable amount of anti—U1 RNP. Treatment of mononuclear cells by cycloheximide resulted in complete inhibition of anti—U1 RNP secretion, which indicates that antibody in the culture supernatants reflects the active biologic phenomenon in vitro. The methods described should be useful in the study of the cellular mechanisms involved in antinu‐clear antibody production of connective tissue diseases.

元の言語English
ページ(範囲)1343-1350
ページ数8
ジャーナルArthritis & Rheumatism
29
発行部数11
DOI
出版物ステータスPublished - 1986 11

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

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