In vitro production of autoantibodies to U1 ribonucleoproteins by peripheral blood mononuclear cells from patients with connective tissue diseases

Y. Ohosone, M. Akizuki, Michito Hirakata, M. Satoh, H. Yamagata, M. Homma

研究成果: Article

7 引用 (Scopus)

抄録

Antibodies to U1 ribonucleoproteins produced by peripheral blood mononuclear cells from patients with connective tissue diseases were measured by a sensitive enzyme-linked immunosorbent assay and by immunoblotting. Mononuclear cells from patients with high-titer serum antibodies spontaneously started secreting IgG anti-U1 RNP antibodies on the second day after culture. The amount of anti-U1 RNP in the culture supernatants reached maximum level on day 8. The most effective production of anti-U1 RNP by B cells was observed when they were cultured in the presence of T cells and adherent cells. Mononuclear cells from patients without anti-U1 RNP antibodies or from normal subjects did not produce a measurable amount of anti-U1 RNP. Treatment of mononuclear cells by cycloheximide resulted in complete inhibition of anti-U1 RNP secretion, which indicates that antibody in the culture supernatants reflects the active biologic phenomenon in vitro. The methods described should be useful in the study of the cellular mechanisms involved in antinuclear antibody production of connective tissue diseases.

元の言語English
ページ(範囲)1343-1350
ページ数8
ジャーナルArthritis and Rheumatism
29
発行部数11
DOI
出版物ステータスPublished - 1986

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Ribonucleoproteins
Connective Tissue Diseases
Autoantibodies
Blood Cells
Antibodies
Biological Phenomena
Antinuclear Antibodies
Cycloheximide
Immunoblotting
Antibody Formation
B-Lymphocytes
Enzyme-Linked Immunosorbent Assay
In Vitro Techniques
T-Lymphocytes
Serum

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

これを引用

In vitro production of autoantibodies to U1 ribonucleoproteins by peripheral blood mononuclear cells from patients with connective tissue diseases. / Ohosone, Y.; Akizuki, M.; Hirakata, Michito; Satoh, M.; Yamagata, H.; Homma, M.

:: Arthritis and Rheumatism, 巻 29, 番号 11, 1986, p. 1343-1350.

研究成果: Article

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AU - Akizuki, M.

AU - Hirakata, Michito

AU - Satoh, M.

AU - Yamagata, H.

AU - Homma, M.

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