In vitro reconstitution of an erythropoietin gene transcription system using its 5′-flanking sequence and a nuclear extract from anemic kidney

Terumasa Tsuchiya, Hirokazu Ochiai, Shinobu Imajoh-Ohmi, Masatsugu Ueda, Toshio Suda, Michio Nakamura, Shiro Kanegasaki

研究成果: Article査読

7 被引用数 (Scopus)

抄録

We have developed an in vitro transcription system for the erythropoietin (Epo) gene. This system uses a plasmid carrying 0.2 kb of 5′-flanking sequence from the human Epo gene, rNTPs and a nuclear extract from mouse kidney. The transcribed RNA was assayed by primer extension with an end-labeled primer complementary to the sequence of the plasmid, dNTPs and reverse transcriptase. The primer extension product corresponding to the transcript was detected on a sequencing gel. The in vitro promoter activity of the Epo 5′-flanking sequence was observed with a nuclear extract from anemic kidney but not with that from normal kidney.

本文言語English
ページ(範囲)137-143
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
182
1
DOI
出版ステータスPublished - 1992 1 15

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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