In vitro selection of GTP-binding proteins by block shuffling of estrogen-receptor fragments

Toru Tsuji, Michiko Onimaru, Nobuhide Doi, Etsuko Miyamoto-Sato, Hideaki Takashima, Hiroshi Yanagawa

研究成果: Article

7 引用 (Scopus)

抜粋

To what extent has alternative splicing contributed to the evolution of protein-function diversity? We previously constructed a pool of block-deletion mutants of the human estrogen receptor α ligand binding domain by random multi-recombinant PCR. Here we performed iterative in vitro selection of GTP-binding proteins by using the library of mRNA-displayed proteins and GTP-affinity chromatography combined with quantitative real-time PCR. We obtained a novel GTP-binding protein with moderate affinity and substrate-specificity. The results of our in vitro simulation imply that alternative splicing may have contributed substantially to the diversification of protein function during evolution.

元の言語English
ページ(範囲)689-693
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
390
発行部数3
DOI
出版物ステータスPublished - 2009 12 18

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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