抄録
Two-gene vectors with positive or positive-negative drug-selectable markers enable the expansion or elimination of gentetically modified cells in vivo. We have established a bicistronic retroviral vector system which utilizes an internal ribosome entry site (IRES) to co-express two independent genes with high efficiency. As a positive-negative (suicide) marker, Herpes simplex virus thymidine kinase was co-expressed with the human multidrug resistance gene, MDR1. Using this vector, almost all the MDR1-transduced cells showed hypersensitivity to a nucleoside analog, ganciclovir. As a dominant selectable marker, the MDR1 gene was co-expressed with α-galactosidase A for the model of gene therapy of Fabry disease. Vincristine selection efficiently enhanced the population of transduced cells expressing the second non-selectable genes. These drug-selectable retroviral vectors could be applicable to the therapy of many diseases.
本文言語 | English |
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ページ(範囲) | 552-556 |
ページ数 | 5 |
ジャーナル | Leukemia |
巻 | 11 |
号 | SUPPL. 3 |
出版ステータス | Published - 1997 1月 1 |
外部発表 | はい |
ASJC Scopus subject areas
- 血液学
- 腫瘍学
- 癌研究