In vivo role of leukocyte ADAM17 in the inflammatory and host responses during E. coli-mediated peritonitis

Chunmei Long, Yue Wang, Amy H. Herrera, Keisuke Horiuchi, Bruce Walcheck

研究成果: Article

49 引用 (Scopus)

抄録

Inflammation is the body's initial response to infection, which is harmful when excessive, as exemplified in sepsis inflammatory syndromes. Ectodomain shedding by the membrane metalloprotease ADAM17 is an emerging regulator of inflammation, as it directs the activity of various inflammatory modulators. At this time, however, little is known about the in vivo function of ADAM17. Here, we show that ADAM17-deficient leukocytes afforded mice a survival benefit following Escherichia coli-mediated peritoneal sepsis, which was associated with a reduction in systemic proinflammatory cytokine levels and bacterial burden. A more rapid yet transitory neutrophil infiltration into the peritoneal cavity of conditional ADAM17 knockout mice was observed when compared with control mice, suggesting a mechanism for their enhanced clearance of bacteria. Preventing the shedding of L-selectin augments neutrophil recruitment, and we show that L-selectin shedding by peritoneal neutrophils in conditional ADAM17 knockout mice was impaired. Moreover, their peritoneal TNF-α levels were markedly lower than control mice following E. coli challenge. These events indicate key molecular processes involved in the altered time course of neutrophil recruitment in conditional ADAM17 knockout mice. Overall, our study provides novel in vivo evidence of the instrumental role of ADAM17 in modulating inflammation and host resistance during Gram-negative bacterial infection.

元の言語English
ページ(範囲)1097-1101
ページ数5
ジャーナルJournal of Leukocyte Biology
87
発行部数6
DOI
出版物ステータスPublished - 2010 6

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Peritonitis
Leukocytes
Escherichia coli
Neutrophil Infiltration
Knockout Mice
L-Selectin
Inflammation
Gram-Negative Bacterial Infections
Systemic Inflammatory Response Syndrome
Peritoneal Cavity
Metalloproteases
ADAM17 Protein
Sepsis
Neutrophils
Cytokines
Bacteria
Membranes
Infection

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

これを引用

In vivo role of leukocyte ADAM17 in the inflammatory and host responses during E. coli-mediated peritonitis. / Long, Chunmei; Wang, Yue; Herrera, Amy H.; Horiuchi, Keisuke; Walcheck, Bruce.

:: Journal of Leukocyte Biology, 巻 87, 番号 6, 06.2010, p. 1097-1101.

研究成果: Article

Long, Chunmei ; Wang, Yue ; Herrera, Amy H. ; Horiuchi, Keisuke ; Walcheck, Bruce. / In vivo role of leukocyte ADAM17 in the inflammatory and host responses during E. coli-mediated peritonitis. :: Journal of Leukocyte Biology. 2010 ; 巻 87, 番号 6. pp. 1097-1101.
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