Incorporation of glycosylated amino acid into protein by an in vitro translation system

Teruhiko Matsubara, Kazutoshi Iijima, Takayoshi Watanabe, Takahiro Hohsaka, Toshinori Sato

研究成果: Article査読

5 被引用数 (Scopus)

抄録

O-GalNAcα-modified proteins are the precursor of mucin-type O-glycosylated proteins. Homogeneously O-glycosylated proteins are required to investigate the biological functions of glycoproteins and to develop biopharmaceuticals. Here we show that the incorporation of GalNAcα-Thr into proteins successfully proceeded by the use of a chemically aminoacylated tRNA. GalNAcα-Thr was chemoenzymatically attached to amber suppressor tRNA and the product was subjected to in vitro translation together with streptavidin mRNA containing the UAG codon. Gel electrophoresis and mass analysis showed that GalNAcα-Thr was successfully incorporated into the N-terminus, although it was not incorporated at the interior. This method will facilitate the preparation of homogeneous GalNAcα-proteins.

本文言語English
ページ(範囲)5634-5636
ページ数3
ジャーナルBioorganic and Medicinal Chemistry Letters
23
20
DOI
出版ステータスPublished - 2013 10 15

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学

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